CJC-1295 peptide: what the human trials measured, DAC vs Mod GRF 1-29, and what was never tested
What 37 indexed publications and 1 randomized trials actually state about cjc-1295, sentence by sentence, each tied to its source and labeled by the kind of evidence it is.
At a glance
What CJC-1295 is and how it acts
CJC-1295 is a peptide in the ghrh analogs class (GHRH receptor agonist, modified GRF(1-29), with or without DAC). Europe PMC indexes 37 publications naming it or a listed alias in a title or abstract, including 1 randomized controlled trials and 1 clinical trials of any design, as of . The strongest evidence tier in that literature is human clinical trials.
This page reports what those studies state, sentence by sentence, with each statement tied to its source and labeled by the kind of evidence it is. It does not recommend use, and it does not translate study doses into anything personal.
What the evidence level means
Publication counts show what is indexed, not how strong the evidence is. The ledger below separates human clinical records from preclinical, analytical, and in-vitro work.
Interpretation boundary: An indexed publication count is a discovery measure. It does not establish efficacy, safety, approval, or a personal treatment plan.
CJC-1295 in two minutes
What it is. Growth-hormone-releasing hormone's active fragment, stabilised at four positions and hooked to albumin after injection, so that one dose raises growth hormone for about a week. Developed by ConjuChem; tested in healthy adults in 2006; abandoned the same year.
What the research actually shows. 37 indexed publications, of which one randomized trial paper, one pulsatility study and one proteomics study in people. A single injection raised growth hormone 2- to 10-fold for six days and IGF-1 1.5- to 3-fold for 9 to 11 days, with a half-life of 5.8 to 8.1 days. Nothing else was measured in people: not fat, muscle, sleep, skin or recovery.
DAC and no DAC. Every human study used the DAC form. 'CJC-1295 without DAC' is Mod GRF 1-29, the same peptide without the albumin hook, and it has never been given to a person in a published study.
Safety record. No serious adverse reactions in 28 to 49 days of healthy-adult trials. One death in the 192-participant phase 2 trial, judged unrelated, after which development stopped. FDA cited heart-rate and vasodilatory reactions in 2023.
Status. Not approved; named on the WADA list under S2; not compoundable under 503A. Paired with ipamorelin in most use, a combination tested only in mice.
Editorial synthesis Written from general pharmacology, the development record and what is commonly reported; not a cited finding. Cited findings on this page carry their own tier.
How it works
Written from general pharmacology and the development record, and marked as editorial synthesis; the measurements behind it are quoted in the biomarker section.
- Editorial synthesis from general knowledge
- Editorial synthesis from general knowledge
- Editorial synthesis from general knowledge
CJC-1295 with DAC vs without DAC (Mod GRF 1-29)
The two products sold under one name are different compounds with different evidence. Only one of them has been in a human study.
| CJC-1295 (with DAC) | Mod GRF 1-29 ('CJC-1295 no DAC') | |
|---|---|---|
| Structure | GRF(1-29) with four substitutions plus an albumin-binding lysine derivative at the C-terminus | GRF(1-29) with the same four substitutions, no albumin-binding group |
| Human studies | Two 2006 trials in healthy adults; one pulsatility study; one proteomics study; a halted 192-participant phase 2 | None under either name |
| Half-life in people | 5.8 to 8.1 days, measured | Not measured; the minutes-long figure quoted is borrowed from GRF(1-29) and sermorelin |
| What a dose does | Raises trough growth hormone for days; pulses unchanged | Presumed to produce a single pulse, as native GHRH does; not shown |
| Doses in studies | 30 to 250 micrograms per kilogram, single; weekly or biweekly repeats | None |
| Why people choose it | Fewer injections | Belief that a pulse is more physiological than a plateau; that belief has not been tested against the DAC form |
The 'no DAC' name is a vendor coinage: ConjuChem's development papers call the unhooked peptide a GRF(1-29) analogue and used it as the starting material. When a page gives a Mod GRF 1-29 half-life, dose or safety profile, it is describing sermorelin's and assuming the four substitutions change nothing but enzyme resistance.
Editorial synthesis Written from general pharmacology, the development record and what is commonly reported; not a cited finding. Cited findings on this page carry their own tier.
What happened in the human studies?
This record's ledger holds 3 primary human studies, of which 2 are trials. Few enough to show in full: each card quotes what its abstract reported about CJC-1295. Read them before any other section on this page.
-
GH secretion was increased after CJC-1295 administration with preserved pulsatility.
Sourcepmid-17018654· quoted verbatim from the abstract -
After a single injection of CJC-1295, there were dose-dependent increases in mean plasma GH concentrations by 2- to 10-fold for 6 d or more and in mean plasma IGF-I concentrations by 1.5- to 3-fold for 9-11 d.
Sourcepmid-16352683· quoted verbatim from the abstract -
Serum proteins displaying significant changes before and after treatment were subsequently identified using mass spectrometry.
Sourcepmid-19386527· quoted verbatim from the abstract
What did the trials find?
Hormone changes in healthy adults over days to weeks. No trial measured muscle, fat, sleep, skin or recovery, which is what the compound circulates for. One row per primary study in this record's ledger. Fields are read from each paper's indexing and abstract. "Analytical method" marks papers about detecting or measuring the compound rather than about its effects.
| Study | Design | n | Species | Dose | Route | Duration | Reported outcome | Tier |
|---|---|---|---|---|---|---|---|---|
| Ionescu 2006 | Clinical trial | — | Humans | — | — | — | GH secretion was increased after CJC-1295 administration with preserved pulsatility. | Human clinical trial |
| Teichman 2006 | Randomized controlled trial | — | Humans | — | subcutaneous | — | After a single injection of CJC-1295, there were dose-dependent increases in mean plasma GH concentrations by 2- to 10-fold for 6 d or more and in mean plasma IGF-I concentrations by 1.5- to 3-fold for 9-11 d. | Human clinical trial |
| Sackmann-Sala 2009 | Human study | — | Humans | — | — | — | Serum proteins displaying significant changes before and after treatment were subsequently identified using mass spectrometry. | Observational, human |
| Thomas 2022 | Analytical method | — | Swine | — | — | — | In contrast to urine, blood analysis essentially relies on the detection of intact peptide hormones, and the expected concentrations are commonly higher in blood samples than in urine. | Animal, preclinical |
| Timms 2019 | Analytical method | — | — | — | — | — | These CJC-1295-protein conjugates have a much greater half-life compared to the unconjugated peptide and are capable of stimulating GH production for more than six days in humans after a single administration. | Animal, preclinical |
| Kwok 2013 | Analytical method | — | — | — | — | — | — | Animal, preclinical |
| Gautam 2009 | Animal study | — | Mice | — | — | — | We report the surprising observation that mutant mice that selectively lack the M(3) muscarinic acetylcholine receptor subtype in the brain (neurons and glial cells; Br-M3-KO mice) showed a dwarf phenotype associated… | Animal, preclinical |
| Alba 2006 | Animal study | — | Mice | — | — | 1 wk; 5 wk | CJC-1295 caused an increase in total pituitary RNA and GH mRNA, suggesting that proliferation of somatotroph cells had occurred, as confirmed by immunohistochemistry images. | Animal, preclinical |
| Jetté 2005 | Animal study | — | Rats | — | — | — | The best compound, CJC-1295, showed a 4-fold increase in GH area under the curve over a 2-h period compared with hGRF(1-29). | Animal, preclinical |
| Uçaktürk 2026 | Analytical method | — | Humans | — | — | — | — | Mechanistic, in vitro |
| Cristea 2023 | Analytical method | — | — | — | — | — | — | Mechanistic, in vitro |
| Coppieters 2022 | Analytical method | — | — | — | — | — | In a comparison with immuno-affinity purification, enhanced recoveries (59 - 115%) and similar sensitivity were achieved, yet at lower operational costs. | Mechanistic, in vitro |
| Danila 2022 | Analytical method | — | — | — | — | — | The affinity profiles of CBD and carboxy-THC are significantly different from the profiles of synthetic GHR mimetics such as CJC-1295 or [D-Arg 1 -D-Phe 5 -D-Trp 7,9 -Leu 11 ]-Substance P peptides, which are the most… | Mechanistic, in vitro |
| Pineau 2016 | Analytical method | — | Humans | — | — | — | — | Mechanistic, in vitro |
| Knoop 2016 | In vitro study | — | Humans | — | — | — | — | Mechanistic, in vitro |
Doses reported in studies
Each entry is a sentence quoted from the study's abstract, with the study's design, sample size and year in front of it. Comparator doses in the same sentence are not attributed to cjc-1295.
- Source
pmid-16352683 - Source
pmid-16352683 - Source
pmid-17018654 - Source
pmid-16822960
Every CJC-1295 dose in the literature, in one table
Every figure here was given in a study, by body weight. The trials measured hormones; none measured the outcomes the compound circulates for.
| Study | Who | Route and dose | Duration | What was measured |
|---|---|---|---|---|
| Teichman 2006, trial 1 | Healthy adults 21 to 61 | Subcutaneous, one of four ascending single doses; 30 and 60 mcg/kg named as best tolerated | 28 days | Growth hormone and IGF-1 peaks and area under the curve; pharmacokinetics; adverse events |
| Teichman 2006, trial 2 | Healthy adults 21 to 61 | Subcutaneous, two or three weekly or biweekly doses | 49 days | IGF-1 above baseline up to 28 days; cumulative effect |
| Ionescu 2006 | Healthy men 20 to 40 | Subcutaneous, 60 or 90 mcg/kg, single | One week | Overnight growth-hormone pulsatility; IGF-1 |
| Sackmann-Sala 2009 | 11 healthy young men | Single injection; dose not stated in the abstract | One week | Serum protein changes |
| ConjuChem phase 2 (secondary reports; not in ledger) | 192 adults with HIV-associated visceral fat | Weekly subcutaneous, ascending; figure reported second-hand, not reproduced | Halted after the 11th weekly dose in one participant | Visceral fat; ended after a death |
| Alba 2006 | One-week-old mice lacking GHRH | 2 micrograms every 24, 48 or 72 hours | Five weeks | Length, weight, bone length, body composition, pituitary GH mRNA |
| Jetté 2005 | Rats | Subcutaneous; dose not stated in the abstract | Single dose; plasma to 72 hours | Growth-hormone area under the curve; albumin binding |
Figures for CJC-1295 that circulate on forums and vendor pages were not taken from these trials, which dosed by body weight for at most 49 days. They are not reproduced here; the table holds every dose a study actually administered.
Editorial synthesis Written from general pharmacology, the development record and what is commonly reported; not a cited finding. Cited findings on this page carry their own tier.
Side effects: what the trials recorded, and what happened in phase 2
The 2006 trials in healthy adults reported no serious adverse reactions over 28 to 49 days. A death in the halted 2006 phase 2 trial and the concerns FDA cited in 2023 are set out below, with their limits. Events and their frequency as each study reported them, with the denominator where the abstract gives one.
- Source
pmid-16352683 - Source
pmid-16352683 - Editorial synthesis from general knowledge · primary document to be added to the ledger
- Editorial synthesis from general knowledge · primary document to be added to the ledger
- Editorial synthesis from general knowledge
Who CJC-1295 is discussed for, and the cautions that recur
Studied: healthy adults aged 20 to 61, and adults with HIV-associated visceral fat in the halted phase 2. Never studied: children, older adults, people with growth-hormone deficiency, athletes, or anyone taking it for months. Community: people seeking fat loss, muscle, sleep or recovery, usually with ipamorelin.
No trial published exclusion criteria in its abstract, so these cautions come from the compound's pharmacology and its record, not from a contraindication list:
- Cardiovascular disease. The one death in the programme was a heart attack in a participant with undiagnosed coronary disease; FDA later cited increased heart rate and vasodilatory reactions. Whether the drug contributed is unknown, which is not the same as known not to.
- Active or recent cancer. IGF-1 stays raised for weeks per dose; no study has looked at what that does to a tumour, in either direction.
- Diabetes and insulin resistance. Growth hormone raises blood glucose; the healthy-adult trials did not report glucose in their abstracts, and the 2026 sports-medicine review lists insulin resistance among documented risks of this peptide class.
- Anyone whose pituitary cannot make growth hormone. A GHRH analogue has nothing to act on; the mouse growth result was in animals lacking GHRH, not growth hormone.
- Women. The 2016 forum study found women themselves worried that doses and cycles circulating were worked out for men; no trial has reported results by sex.
- Tested athletes. Named on the WADA list; detectable in urine and blood by published methods.
Editorial synthesis Written from general pharmacology, the development record and what is commonly reported; not a cited finding. Cited findings on this page carry their own tier.
Biomarkers measured in studies
Laboratory and clinical measures the studies report tracking, including which hormones did and did not change.
-
A synthetic GHRH analog (CJC-1295) that binds permanently to endogenous albumin after injection (half-life = 8 d) stimulates GH and IGF-I secretion in several animal species and in normal human subjects and enhances growth in rats.
Sourcepmid-17018654· quoted verbatim from the abstract -
The main outcome measures were peak concentrations and area under the curve of GH and IGF-I; standard pharmacokinetic parameters were used for CJC-1295.
Sourcepmid-16352683· quoted verbatim from the abstract -
Serum GH and IGF-1 levels have been shown to increase with administration of GHRH or CJC-1295, a long-acting GHRH analog.
Sourcepmid-19386527· quoted verbatim from the abstract -
Co-extraction and analysis of several different peptides such as insulins (human, lispro, aspart, glulisine, tresiba, detemir, glargine, bovine insulin and porcine insulin), growth hormone releasing hormones (sermorelin, CJC-1295 and tesamorelin), insulin-like growth factors (long-R 3 -IGF-I, R 3 -IGF-I and Des 1-3 -IGF-I) and mechano growth factors (human MGF and MGF-Goldspink) with criteria that fulfil the requirements of the WADA documents (TD2022 MRPL) for doping controls.
Sourcepmid-38716080· quoted verbatim from the abstract -
CJC-1295 is a peptide-based drug that stimulates the production of growth hormone (GH) from the pituitary gland.
Sourcepmid-30938069· quoted verbatim from the abstract -
Remarkably, treatment of Br-M3-KO mice with CJC-1295, a synthetic GH-releasing hormone (GHRH) analog, rescued the growth deficit displayed by Br-M3-KO mice by restoring normal pituitary size and normal serum GH and IGF-1 levels.
Sourcepmid-19332789· quoted verbatim from the abstract
Reported timelines
Onset, peak and duration figures as each study reported them.
- Source
pmid-16352683 - Source
pmid-16352683 - Source
pmid-17018654 - Source
pmid-15817669
What is measured over time, and what is not
The hormone timeline is unusually well described for an unapproved peptide. After one injection, growth hormone rose within the first day and stayed 2- to 10-fold above baseline for six days or more; IGF-1 rose more slowly and stayed 1.5- to 3-fold above baseline for 9 to 11 days; with repeated doses it remained raised for up to 28 days and accumulated. In rats the peptide was on albumin within 15 minutes.
What was not measured is everything a person would notice. No trial recorded sleep, body composition, strength, skin or recovery at any time point, and nothing was measured after the 49-day trial ended. 'How long does it take to work' therefore has a hormonal answer, days, and no clinical answer at all.
Editorial synthesis Written from general pharmacology, the development record and what is commonly reported; not a cited finding. Cited findings on this page carry their own tier.
Study durations
Treatment and follow-up periods as stated in each abstract.
- Source
pmid-16352683 - Source
pmid-17018654 - Source
pmid-19386527 - Source
pmid-16822960
Routes studied
Routes of administration named in each study.
- administration by subcutaneous route reported
CJC-1295 or placebo was administered sc in one of four ascending single doses in the first study and in two or three weekly or biweekly doses in the second study.
Sourcepmid-16352683· quoted verbatim from the abstract
Weight-normalized doses, as published
Per-kilogram figures exactly as each study published them, for the species it studied.
- Source
pmid-16352683 - Source
pmid-17018654
Weight-normalized figures above are reproduced exactly as each study published them, for the species it studied. Dose does not scale linearly with body mass between species: metabolic rate, clearance and receptor density differ, and regulatory guidance uses allometric, not proportional, conversion. A mg/kg figure from a rat study is a fact about that rat study and nothing else.
What people report outside the literature
Forum reports are experiences, not evidence. One indexed study analysed them and is quoted here; the doses people describe are not reproduced. What circulates in user communities and clinic marketing, reported so a reader knows the figures and their origin. None of it has been tested in a trial, and none of it is a suggestion.
- Source
pmid-26771670 - Editorial synthesis from general knowledge
Reconstitution mathematics
Lyophilised peptide is supplied as a mass in a vial and dissolved in a stated volume of diluent. Two figures follow from that and nothing else:
- Concentration = mass in vial ÷ diluent volume. A 5 mg vial in 2 mL is 2.5 mg/mL, or 2,500 mcg/mL.
- Volume for a given mass = that mass ÷ concentration. 250 mcg at 2,500 mcg/mL is 0.1 mL. On a U-100 insulin syringe, where 1 mL is 100 units, that is 10 units.
Study doses on this page are quoted as published, in the units the authors used. Converting between mcg, mg and IU, or between a mass and a syringe volume, is arithmetic; deciding a mass is not, and this page does not do it. The reconstitution calculator performs the conversion with the formula shown.
Reading a certificate of analysis
A certificate of analysis is a laboratory report on one batch. Reading it means checking four things in order.
- Identity. Mass spectrometry should report an observed molecular mass matching the peptide's theoretical mass within the instrument's stated tolerance. A certificate with no mass spectrometry, or one that lists only the expected value, has not confirmed identity.
- Purity. HPLC purity is the area of the main peak as a share of all peaks, at a stated wavelength. It says nothing about what the other peaks are, and it is not a measure of quantity.
- Quantity. Net peptide content, by amino acid analysis or UV, is the fraction of the vial's mass that is peptide rather than counter-ions and water. A 5 mg vial at 80% net content holds 4 mg of peptide, and every concentration figure above changes accordingly.
- Provenance. The batch number on the certificate should match the vial, the laboratory should be named and independent of the seller, and the test date should be recent.
This page rates no supplier and links to none. It describes how to read the document.
Equipment described in studies
Published human studies of injectable peptides report the same small set of materials: single-use insulin syringes with fixed needles, most often U-100 with 29 to 31 gauge needles; bacteriostatic water or sterile water for injection as diluent, with the choice stated in the methods; alcohol swabs; and refrigerated storage of reconstituted solution. Animal studies more often report intraperitoneal or intragastric administration and state the vehicle used. Where a study in this record's ledger names its materials or vehicle, that sentence appears under storage or routes above; where it does not, nothing is assumed here.
Storage and handling
No storage or stability study of CJC-1295 is indexed. Vendor sheets follow general practice for lyophilised peptides, refrigerated as powder and colder once in solution. The compound's albumin-reactive group is designed to react with thiols, which is a reason the manufacturer's handling instructions, if a vial has them, matter more than for an ordinary peptide; nothing about that has been published.
Editorial synthesis Written from general pharmacology, the development record and what is commonly reported; not a cited finding. Cited findings on this page carry their own tier.
Common mistakes in how CJC-1295 is discussed
- Giving Mod GRF 1-29 a half-life and a dose. The 'no DAC' compound has no human data. Its figures are sermorelin's, relabelled.
- Turning hormone results into body results. The trials showed growth hormone and IGF-1 rising. Fat loss, muscle, sleep and healing were never measured in any human study of this compound.
- Quoting milligram doses as if they were trial doses. The trials dosed by body weight for at most 49 days. The weekly and daily figures that circulate come from forums.
- Treating the phase 2 death as settled either way. One physician judged it unrelated; no further trial tested that. It is an open item, and the only long-exposure safety datum that exists.
- Calling it 'FDA category 2'. It was, from September 2023 to September 2024, then removed because the nominators withdrew it. It now sits on no category, which is worse for compounding, not better.
- Reading the stack as tested. CJC-1295 with ipamorelin has one mouse study of muscle under steroid-induced wasting. The pairing rationale is pharmacological, not clinical.
Editorial synthesis Written from general pharmacology, the development record and what is commonly reported; not a cited finding. Cited findings on this page carry their own tier.
CJC-1295 vs sermorelin, tesamorelin and ipamorelin
| Compound | What it is | Human evidence | Half-life in people | Status |
|---|---|---|---|---|
| CJC-1295 | GRF(1-29) analogue, albumin-bound (DAC) | Two 2006 trials in healthy adults; halted phase 2 | 5.8 to 8.1 days | Not approved; WADA S2 |
| Sermorelin | GRF(1-29) acetate, unmodified; 579 indexed publications | 39 randomized trials; 21 human studies in its ledger | Minutes | Approved 1990 for paediatric GH deficiency; later withdrawn from the US market; WADA S2 |
| Tesamorelin | GRF(1-44) with a stabilising group; 115 indexed publications | 22 randomized trials; 20 human studies in its ledger | Under an hour | Approved 2010 for HIV-associated abdominal fat; WADA S2 |
| Ipamorelin | Ghrelin-receptor agonist, a different receptor; 63 indexed publications | 2 randomized trials; 6 human studies in its ledger | About two hours | Not approved; WADA S2 |
Tesamorelin is the closest comparator: a stabilised GHRH analogue that completed development for the indication ConjuChem was chasing. The direct comparisons this site holds are ipamorelin vs sermorelin and sermorelin vs tesamorelin; the pairing with ipamorelin has its own stack page.
Editorial synthesis Written from general pharmacology, the development record and what is commonly reported; not a cited finding. Cited findings on this page carry their own tier.
Other compounds in its class
Same class in the registry: Sermorelin, Tesamorelin. Each row shows what that compound's own record states; nothing is inferred across rows.
| Compound | Tier | Publications | RCTs | Record |
|---|---|---|---|---|
| CJC-1295 | Human clinical trial | 37 | 1 | draft |
| Sermorelin | Approved label | 579 | 39 | draft |
| Tesamorelin | Approved label | 115 | 22 | draft |
CJC-1295 with ipamorelin: what has been tested
One mouse study tested CJC-1295 with ipamorelin. No human study has. The stack page holds the detail; this section says only what the combination evidence is.
Whether any indexed study tested CJC-1295 together with the compounds it is commonly combined with. A count of zero is the finding, not a gap in this page.
- 3 indexed studies mention CJC-1295 together with Ipamorelin. CJC-1295 + ipamorelin
Regulatory status
Registry facts only. Jurisdiction-level status, compounding categories and sport prohibitions need their own cited documents before they appear here.
- Editorial synthesis from general knowledge · primary document to be added to the ledger
Open questions and limitations
What is genuinely unknown, stated plainly. A missing field is not evidence that the outcome is safe, effective, or established.
- The halted 2006 phase 2 trial (HIV-associated visceral fat, 192 participants by secondary reports) was never published; its adverse-event data are the only long-exposure safety data that exist and are not available.
- No study in this record's ledger reports dose-escalation schedules for CJC-1295.
- No study in this record's ledger reports exclusion criteria for CJC-1295.
Questions people ask
What is CJC-1295?
CJC-1295 is a synthetic analogue of the first 29 amino acids of growth-hormone-releasing hormone, altered at four positions to resist breakdown and fitted with a linker (the Drug Affinity Complex, DAC) that binds it to albumin in the blood after injection. That binding stretches its half-life from minutes to about a week. It was developed by ConjuChem and tested in healthy adults in 2006; development stopped the same year.
Does CJC-1295 help with weight loss?
No human trial measured fat mass or weight. The 2006 trials measured growth hormone and IGF-1 only. In mice lacking GHRH, daily CJC-1295 kept body composition normal, which is a growth result in a deficient animal, not a fat-loss result. Weight loss is the commonest reason women on forums gave for using it, according to the 2016 netnography, and it has not been tested.
Is CJC-1295 banned in sport?
Yes. The WADA Prohibited List names CJC-1295 as an example under S2, growth hormone releasing hormone and its analogues, prohibited at all times in and out of competition. Detection methods in urine and blood make up a large share of its indexed literature.
Does CJC-1295 cause cancer?
No study has tested it. The concern is indirect: CJC-1295 raises IGF-1 for days to weeks, and sustained IGF-1 elevation is a theoretical risk for cell proliferation. The healthy-adult trials lasted at most 49 days and measured hormones, not tumours, so the question is open rather than answered either way.
What is the half-life of CJC-1295?
An estimated 5.8 to 8.1 days in healthy adults after a single subcutaneous injection, from the 2006 pharmacokinetic study; a second trial that year used a figure of 8 days. That is the DAC form, the only one measured in people. The short half-life quoted for CJC-1295 without DAC is borrowed from GRF(1-29) and sermorelin, not measured for that compound.
Is CJC-1295 FDA approved?
No. It never completed development. In September 2023 FDA placed it in category 2 of the interim 503A bulk-substances list, citing safety concerns, and in September 2024 removed it from that list after the nominators withdrew it, which leaves it on no category and not eligible for compounding. Check the live list; the position has moved twice in two years.
Does CJC-1295 increase testosterone?
No trial measured it, and there is no mechanism for it to. CJC-1295 acts on the GHRH receptor of the pituitary cells that make growth hormone; testosterone is driven by a different pituitary axis. Claims that it raises testosterone come from stacking discussions, not from any study.
Does CJC-1295 increase height?
Not in adults, whose growth plates are closed. The growth result comes from one-week-old mice lacking GHRH, in which daily CJC-1295 normalised length and weight. No child has been given it in a published study, and no human growth data exist.
What are the downsides of CJC-1295?
Sustained IGF-1 elevation of unknown long-term effect; a phase 2 programme halted after a death, judged unrelated by the attending physician but never followed by another trial; FDA's 2023 citation of increased heart rate and systemic vasodilatory reactions; prohibition in sport; and the fact that nothing it circulates for has been measured in a human study.
Has CJC-1295 been tested in humans?
Yes, more than most unapproved peptides. Two randomized, placebo-controlled trials in healthy adults aged 21 to 61 ran for 28 and 49 days in 2006, a pulsatility study gave healthy men one injection, and a proteomics study followed 11 men for a week. A 192-participant phase 2 trial in HIV-associated visceral fat was halted in 2006 and never published in full.
What did the trials actually show?
That one subcutaneous dose raises growth hormone 2- to 10-fold for six days or more and IGF-1 1.5- to 3-fold for 9 to 11 days, that repeated doses keep IGF-1 up for 28 days, and that growth-hormone pulses keep their rhythm while the troughs between them rise. They showed nothing about body composition, sleep or recovery, because those were not measured.
What is the difference between CJC-1295 with DAC and without DAC?
The DAC is the albumin-binding group that gives the compound its week-long half-life. 'Without DAC' is Mod GRF 1-29, the same stabilised GRF(1-29) without that group. Every human study used the DAC form; Mod GRF 1-29 has none, and its short half-life and daily dosing are inferred from sermorelin.
Is there a CJC-1295 dose?
The trials dosed by body weight: four ascending single doses with 30 and 60 micrograms per kilogram named as best tolerated, 60 or 90 micrograms per kilogram in the pulsatility study, and weekly or biweekly repeats for up to 49 days. No dose was established for any use, and the milligram figures that circulate did not come from these trials.
What are the side effects of CJC-1295?
The healthy-adult trials reported no serious adverse reactions and called the lower doses best tolerated. Beyond that, the record is one death in the halted phase 2 trial, judged unrelated by the attending physician, and FDA's 2023 citation of increased heart rate and systemic vasodilatory reactions. Common complaints on forums are flushing, headache, water retention and injection-site reactions.
Why did development of CJC-1295 stop?
ConjuChem halted its phase 2 trial in July 2006 after a participant died of a heart attack two hours after an eleventh weekly injection. The physician attributed it to undiagnosed coronary disease, but the company ended the programme as a precaution and no one has picked the compound up since.
Does CJC-1295 need to be taken with ipamorelin?
No study says so. The pairing rests on pharmacology: CJC-1295 acts on the GHRH receptor and ipamorelin on the ghrelin receptor, and the two pathways are additive in acute growth-hormone tests of other agents. The only study of the pair is in mice with steroid-induced muscle loss. The stack page holds the detail.
Is CJC-1295 an approved medicine?
No, anywhere. It reached phase 2 and stopped. In the United States it is sold as a research chemical, was on FDA's 503A category 2 list from September 2023 to September 2024, and is now on no category, so it cannot lawfully be compounded.
How is CJC-1295 detected in doping control?
By liquid chromatography and mass spectrometry in urine and plasma, with immunoaffinity or ultrafiltration clean-up. Detection papers, including one in horse plasma, make up about a third of the compound's indexed literature, which tells you where the research money has gone since 2006.
Which species has CJC-1295 been studied in?
Humans, in the 2006 trials; rats, in the 2005 development paper; mice lacking GHRH, in the 2006 growth study; and horses and pigs, in doping-detection work. The stack with ipamorelin was tested in mice.
What is Mod GRF 1-29?
Modified GRF(1-29): GRF(1-29) with D-alanine, glutamine, alanine and leucine substituted at positions 2, 8, 15 and 27 so that DPP-IV cannot degrade it. It is the intermediate from which CJC-1295 was built and is sold as 'CJC-1295 no DAC'. It has no human study of its own.
What did the mouse growth study show?
One-week-old mice lacking GHRH given 2 micrograms of CJC-1295 daily for five weeks grew to normal weight and length with normal body composition; every 48 or 72 hours worked less well. Their pituitaries made more growth-hormone mRNA. It is a replacement result in a deficient juvenile animal, not a result about adults.
Sources
Full citations. Every claim above links to one of these by its id.
- 1Peptide Supplements and Their Therapeutic Applications in Sports Medicine.
pmid-42578445· · peer-reviewed - 2
- 3Evaluation of Research Grade Peptides Marketed Directly to Consumers Reveals Extensive Variability in Purity and Measured Abundance
doi-10-20944-preprints202604-1748-v1· · preprint - 4Safety and Efficacy of Approved and Unapproved Peptide Therapies for Musculoskeletal Injuries and Athletic Performance
doi-10-20944-preprints202512-1011-v3· · preprint - 5Therapeutic peptides in gerontology: mechanisms and applications for healthy aging.
pmid-42021992· · peer-reviewed - 6Injectable Peptide Therapy: A Primer for Orthopaedic and Sports Medicine Physicians.
pmid-41476424· · peer-reviewed - 7Safety and Efficacy of Approved and Unapproved Peptide Therapies for Musculoskeletal Injuries and Athletic Performance
doi-10-20944-preprints202512-1011-v1· · preprint - 8
- 9Cationic exchange SPE combined with triple quadrupole UHPLC-MS/MS for detection of GHRHs in urine samples.
pmid-37806509· · peer-reviewed - 10Probing for peptidic drugs (2-10 kDa) in doping control blood samples.
pmid-38716080· · peer-reviewed - 11
- 12
Show the remaining 12 sources
- 13A method for confirming CJC-1295 abuse in equine plasma samples by LC-MS/MS.
pmid-30938069· · peer-reviewed - 14The study of doping market: How to produce intelligence from Internet forums.
pmid-27710891· · peer-reviewed - 15
- 16Netnography of Female Use of the Synthetic Growth Hormone CJC-1295: Pulses and Potions.
pmid-26771670· · peer-reviewed - 17Doping control analysis of seven bioactive peptides in horse plasma by liquid chromatography-mass spectrometry.
pmid-23318763· · peer-reviewed - 18Immunoaffinity purification of peptide hormones prior to liquid chromatography-mass spectrometry in doping controls.
pmid-21871962· · peer-reviewed - 19
- 20Neuronal M3 muscarinic acetylcholine receptors are essential for somatotroph proliferation and normal somatic growth.
pmid-19332789· · peer-reviewed - 21
- 22
- 23
- 24
Reference card
- Compound
- CJC-1295, ghrh analogs
- Evidence tier
- Human clinical trial
- Indexed publications
- 37 · 1 RCTs · 1 other clinical trials
- Approval
- no registered development programme found
- Routes reported
- subcutaneous
- Reviewed
- Adam Mirando, PharmD,
Study figures are as published in each source and are not recommendations. Print or save this card with its date.
Last reviewed and what changed
Newest first. These are the record's own revision dates, and the same dates feed the sitemap.
- · Reviewed by Adam Mirando, PharmD, on 2026-09-25.
- · Label correction: 8 evidence rows built from detection and doping-control papers carried study designs such as animal or in-vitro work. They are relabelled Analytical method, the label scripts/draft_claims.py has applied since the rule was added; these rows predate it. pmid-38716080 (Animal study -> Analytical method); pmid-30938069 (Animal study -> Analytical method); pmid-23318763 (Animal study -> Analytical method); pmid-41138283 (In vitro study -> Analytical method); pmid-37806509 (In vitro study -> Analytical method); pmid-35298973 (In vitro study -> Analytical method); pmid-34736642 (In vitro study -> Analytical method); pmid-27710891 (In vitro study -> Analytical method)
- · Misattribution check: evidence_table (pmid-42578445): a review's collective sentence about six peptides is not one compound's evidence row. The sources stay in the ledger; only the claims that put another agent's result under this compound were removed.
- · Written under the sequencing rule after research/intents/cjc-1295.json: guide (8 sections incl. DAC vs Mod GRF 1-29), FAQ to 12 plus 11 from the map, extractive dose, weight-normalized, duration and timeline claims written by hand from the abstracts (the drafter's regex missed 'microg/kg'), mechanism, reported-use (netnography quoted), regulatory and adverse-event claims; two misdrafted interactions claims removed; intent-driven H1 and title. Triage: guide dose table renamed so it does not duplicate the claims section's H2.
- · Claims drafted extractively from 21 ledger sources by scripts/draft_claims.py: 17 claims, 16 evidence-table rows. Status researched -> draft.
- · Claims drafted extractively from 21 ledger sources by scripts/draft_claims.py: 23 claims, 16 evidence-table rows. Status researched -> draft.
- · Claims drafted extractively from 21 ledger sources by scripts/draft_claims.py: 24 claims, 16 evidence-table rows. Status researched -> draft.
- · Metadata refreshed by scripts/fetch_evidence.py --refresh-meta: tier animal-preclinical -> human-clinical-trial.
- · Evidence fetched from Europe PMC and ChEMBL by scripts/fetch_evidence.py; claims not yet written.