AOD-9604 dosage calculator
Enter a vial and a dose and this returns the concentration, the volume and the syringe units. Underneath it, the same arithmetic worked out in advance for common vials, and the doses published studies of AOD-9604 actually used, every one quoted and cited. It converts numbers. It does not suggest what to take.
Work out concentration and what to draw
Two divisions, both shown. Concentration = mass in the vial ÷ diluent volume. Volume to draw = mass you want ÷ that concentration. Syringe units are that volume × the units per millilitre on your syringe's own scale.
Worked example: 5 mg in 2 mL is 2,500 mcg/mL, so 250 mcg is 0.1 mL, which is 10 units on a U-100 syringe and 4 units on a U-40. The scale is printed on the barrel; reading a U-40 syringe as if it were U-100 is a two-and-a-half-fold error.
AOD-9604 reconstitution chart
Units on a standard U-100 insulin syringe, where 100 units is 1 mL. The rows are the absolute doses quoted in studies of AOD-9604, from 250 mcg to 54 mg, so this chart is specific to this compound rather than a generic ladder. More diluent means more units for the same dose, which is easier to measure accurately and empties the vial no faster.
| Dose | 1 mL diluent (2,000 mcg/mL) | 2 mL diluent (1,000 mcg/mL) | 3 mL diluent (667 mcg/mL) |
|---|---|---|---|
| 250 mcg | 12.5 | 25 | 37.5 |
| 1 mg | 50 | 100 | 150 >1 mL |
| 6 mg | 300 >1 mL | 600 >1 mL | 900 >1 mL |
| 30 mg | 1,500 >1 mL | 3,000 >1 mL | 4,500 >1 mL |
| 54 mg | 2,700 >1 mL | 5,400 >1 mL | 8,100 >1 mL |
| Dose | 1 mL diluent (5,000 mcg/mL) | 2 mL diluent (2,500 mcg/mL) | 3 mL diluent (1,667 mcg/mL) |
|---|---|---|---|
| 250 mcg | 5 | 10 | 15 |
| 1 mg | 20 | 40 | 60 |
| 6 mg | 120 >1 mL | 240 >1 mL | 360 >1 mL |
| 30 mg | 600 >1 mL | 1,200 >1 mL | 1,800 >1 mL |
| 54 mg | 1,080 >1 mL | 2,160 >1 mL | 3,240 >1 mL |
| Dose | 1 mL diluent (10,000 mcg/mL) | 2 mL diluent (5,000 mcg/mL) | 3 mL diluent (3,333 mcg/mL) |
|---|---|---|---|
| 250 mcg | 2.5 | 5 | 7.5 |
| 1 mg | 10 | 20 | 30 |
| 6 mg | 60 | 120 >1 mL | 180 >1 mL |
| 30 mg | 300 >1 mL | 600 >1 mL | 900 >1 mL |
| 54 mg | 540 >1 mL | 1,080 >1 mL | 1,620 >1 mL |
Read a figure off this chart only if it matches the vial in front of you. A vial labelled a different strength, or reconstituted with a different volume, gives a different number for the same dose, and that is the single most common way these calculations go wrong.
Doses in published studies of AOD-9604
Each row is quoted from a paper in this compound's source ledger and carries the kind of evidence it came from. 4 of 6 come from human studies; the rest are animal or laboratory work.
| Evidence | Dose as the paper states it | Source |
|---|---|---|
| Human clinical trial | Intravenous doses of 25 to 400 micrograms per kilogram in the first two trials; oral doses of 0.25 to 54 mg a day in the last four. | doi-10-4021-jem157w |
| Human clinical trial | The 24-week phase 2b tested 0.25, 0.5 and 1 mg tablets daily in 502 obese adults. | doi-10-4021-jem157w |
| Human clinical trial | The 12-week phase 2b tested 1 to 30 mg capsules daily in 300 obese adults. | doi-10-4021-jem157w |
| Human clinical trial | Single oral doses of 9, 27 and 54 mg, and seven days of the same doses, in the phase 2a studies. | doi-10-4021-jem157w |
| Animal, preclinical | Obese Zucker rats received 500 micrograms per kilogram orally each day for 19 days and gained less than half the weight of controls. | pmid-11146367 |
| Animal, preclinical | Rabbits with collagenase-induced knee arthritis received weekly intra-articular injections of 0.25 mg, alone or with 6 mg hyaluronic acid. | pmid-26275694 |
Weight-normalised doses, and why they are not on the chart
Some studies state a dose per kilogram. Those figures are deliberately absent from the chart above, because multiplying one by a body weight produces a number that looks like a dose and is not one. Interspecies scaling is not arithmetic: a rat milligram-per-kilogram figure does not become a human dose by multiplication.
- 25 to 400 micrograms per kilogram, intravenous, single doses, in healthy and obese men. doi-10-4021-jem157w
- 500 micrograms per kilogram a day, oral, in obese rats. pmid-11146367
How long studies ran, and how far a vial goes
A vial lasts for a number of draws, not a number of weeks: divide the mass in the vial by the mass per draw and round down, which the vial planner does. How long that represents depends on frequency. For context, these are the durations studies of AOD-9604 actually ran for.
- Single doses in the first three trials; seven days in the fourth; 12 weeks and 24 weeks (with a four-week run-in and 30-day follow-up) in the two phase 2b trials. doi-10-4021-jem157w
- Animal study, rabbits, n=32, 2015: durations stated: 7 weeks. Weekly injections of 0.6 mL saline (Group 1), 6 mg HA (Group 2), 0.25 mg AOD9604 (Group 3), and 0.25 mg AOD9604 with 6 mg HA (Group 4) were administered for 4-7 weeks after the first intra-articular collagenase injection. pmid-26275694
- Animal study, rats, 2000: durations stated: 19 days. Daily treatment with an oral dose of AOD9604 of 500 microg/kg body weight for 19 days reduced over 50% (15.8 +/- 0.6 vs. 35.6 +/- 0.8 g) body weight gain of the animals in comparison with the control. pmid-11146367
Storage and stability, as published
Reconstitution starts a clock. These are the storage and stability conditions stated in papers in this ledger. They describe what the study did, which is not the same as a shelf life for a vial in your fridge.
- In vitro study, humans, 2026: At 4 and 22 °C, alexamorelin, AOD9604, buserelin, hGH 176-191, kisspeptin-10 and LHRH were extensively degraded after one week in serum and plasma, whereas BPC-157, TB500, vasopressin, lypressin, and terlipressin showed complete degradation only in serum. pmid-42328738
How AOD-9604 was given
The route changes what a dose means. The same number given intravenously and subcutaneously is not the same exposure.
- Animal study, mice, 2001: administration by intraperitoneal route reported. Here we describe how hGH and AOD9604 can reduce body weight and body fat in obese mice following 14 d of chronic ip administration. pmid-11713213
- Animal study, rats, 2000: administration by oral route reported. Daily treatment with an oral dose of AOD9604 of 500 microg/kg body weight for 19 days reduced over 50% (15.8 +/- 0.6 vs. 35.6 +/- 0.8 g) body weight gain of the animals in comparison with the control. pmid-11146367
- In vitro study, humans, 2003: administration by inhaled, oral route reported. This issue focuses on the following selection of drugs: Abetimus sodium, adefovir dipivoxil, AGI-1067, alefacept, alemtuzumab, ALVAC-p53, aminolevulinic acid hydrochloride, aminolevulinic acid methyl ester, Anti-CTLA-4 Mab, AOD-9604, apafant, aprinocarsen… pmid-14571286
Questions people ask
- How many units is 250 mcg of AOD-9604?
- It depends entirely on how the vial was reconstituted. In a 5 mg vial with 2 mL of diluent it is 10 units on a U-100 syringe; with 1 mL it is 5. The chart above gives the rest.
- Does adding more diluent waste the peptide?
- No. The mass in the vial does not change. More diluent spreads the same mass through more liquid, so each dose is a larger, easier-to-measure volume and the vial yields exactly as many doses as before.
- Why does this page not recommend a dose?
- Because a dose is a clinical decision rather than a division. It depends on why someone is taking something, what else they take and how their kidneys and liver are working, none of which is knowable from a number typed into a box.
- Where do the doses in the table come from?
- Each is quoted from a specific paper indexed in Europe PMC, linked in the source column, and labelled with the kind of study it came from. The full evidence is on the AOD-9604 record.
What this calculator will not do
It will not take a body weight and return a milligram figure, and it will not tell you how often to draw. The table above is the published record, not a menu. A dose that appeared in a trial appeared under supervision, with monitoring, in people selected for that trial.
Other calculators
- Generic reconstitution calculatorThe same arithmetic, without a compound attached
- Unit convertermg, mcg, ng and syringe units
- Vial plannerDraws per vial and how long it lasts
- All compound calculatorsEvery compound with published dose figures